Biochem/physiol Actions

Target Sub-Family: AGC

Protein Target: Akt1/PKBα

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Recombinant full-length human Akt1, containing N-terminal His6 tag.

Product Source: Expressed by baculovirus in Sf21 insect cells

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250µg size, please inquire for pricing and availability.

Physical form

Supplied in 50mM Tris/HCl pH7.5, 150mM NaCl, 150mM imidazole, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.07% 2-mercaptoethanol, 0.02% Brij 35, 270mM sucrose. Frozen solution.

Ni²⁺/NTA and glutathione agarose

Quality

Routinely evaluated by phosphorylation of Crosstide. Please refer to the Certificate of Analysis for more information.

Storage and Stability

1 year at -70°C